Mutations in Pea seedborne mosaic virus Genome-Linked Protein VPg Alter Pathotype-Specific Virulence in Pisum sativum
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منابع مشابه
Polyadenylate-Binding Protein from Pea (Pisum sativum)'
A polyadenylate-binding protein (PABP) was purified from cellfree extracts prepared from pea seedlings (Pisum sativum) by ammonium sulfate precipitation and Affi-Gel Blue and polyadenylate-Sepharose 4B affinity chromatography. The final preparation from polyadenylate-Sepharose 4B columns contained a single 70-kilodalton polypeptide with high polyadenylate-binding activity. The purified protein ...
متن کاملInhibition of pokeweed antiviral protein (PAP) by turnip mosaic virus genome-linked protein (VPg).
Pokeweed antiviral protein (PAP) from Phytolacca americana is a ribosome-inactivating protein (RIP) and an RNA N-glycosidase that removes specific purine residues from the sarcin/ricin loop of large rRNA, arresting protein synthesis at the translocation step. PAP is also a cap-binding protein and is a potent antiviral agent against many plant, animal, and human viruses. To elucidate the mechani...
متن کاملCharacterization of pea (Pisum sativum) seed protein fractions.
BACKGROUND Legume seed proteins have to be chemically characterized in order to properly link their nutritional effects with their chemical structure. RESULTS Vicilin and albumin fractions devoid of cross-contamination, as assessed by mass peptide fingerprinting analysis, were obtained from defatted pea (Pisum sativum cv. Bilbo) meal. The extracted protein fractions contained 56.7-67.7 g non-...
متن کاملPlasma Membrane Protein from Etiolated Pea (Pisum sativum L.) Seedlings1
When crude microsomal membranes from apical stem segments of etiolated Pisum sativum L. cv Alaska are mixed in vitro with 'y-[32P]ATP, a phosphorylated band of apparent molecular mass 120 kilodaltons can be detected on autoradiographs of sodium dodecyl sulfate electrophoresis gels. If the stem sections are exposed to blue light immediately prior to membrane isolation, this band is not evident. ...
متن کاملPea, Pisum sativum, and Its Anticancer Activity
Pisum sativum (Family: Fabaceae), as known as green pea or garden pea, has long been important in diet due to its content of fiber, protein, starch, trace elements, and many phytochemical substances. It has been shown to possess antibacterial, antidiabetic, antifungal, anti-inflammatory, antihypercholesterolemia, and antioxidant activities and also shown anticancer property. Its nonnutritive bi...
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ژورنال
عنوان ژورنال: Molecular Plant-Microbe Interactions®
سال: 2001
ISSN: 0894-0282,1943-7706
DOI: 10.1094/mpmi.2001.14.6.707